Difference between revisions of "Part:BBa E0040:Experience"
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E0040 contains 2 dpnI sites.  This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest.  For this to work the cell strain that the template was purified from must be dam methylase+.
 
E0040 contains 2 dpnI sites.  This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest.  For this to work the cell strain that the template was purified from must be dam methylase+.
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{|width='80%' style='border:1px solid gray'
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<partinfo>BBa_I15010 AddReview 0</partinfo>
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<I>iGEM11_WHU_China</I>
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This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks.
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Revision as of 09:09, 1 October 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_E0040

User Reviews

UNIQa8014a66bf81fe24-partinfo-00000000-QINU

•••••

Antiquity

This review comes from the old result system and indicates that this part worked in some test.

UNIQa8014a66bf81fe24-partinfo-00000002-QINU

E0040 contains 2 dpnI sites. This is useful when using a part containing E0040 as a template for PCR because you can cut up the template via a dpnI digest. For this to work the cell strain that the template was purified from must be dam methylase+.

iGEM11_WHU_China

This part was used by WHU_China team as a reporter of long-term oscillator. Information from the website of this part shows that its length is about 720bp; however, the result of restriction enzymes cleavage experiment showed that it is actually about 1kb. And after several attempts, we failed to assemble this part with other biobricks.

;