Difference between revisions of "Part:BBa K646001:Design"

 
(Design Notes)
 
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<partinfo>BBa_K646001 short</partinfo>
 
<partinfo>BBa_K646001 short</partinfo>
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===Design Notes===
 
===Design Notes===
Contains two internal Pst1 RE sites.
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Contains two internal Pst1 restriction endonuclease sites that was removed via site directed mutagenesis from ctgcag to cttcag.
 
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===Source===
 
===Source===

Latest revision as of 22:22, 30 September 2011

Agrobacterium tumefaciens Bacteriophotochrome (Rhizobium radiobacter)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1867
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1196
    Illegal NgoMIV site found at 1694
    Illegal AgeI site found at 1759
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contains two internal Pst1 restriction endonuclease sites that was removed via site directed mutagenesis from ctgcag to cttcag.

Source

From 2010 Macquarie Team, isolated from Genomic DNA

References