Difference between revisions of "Part:BBa K560000"

Revision as of 15:03, 30 September 2011

Blue Light Sensor+PtrpL+mRFP1

Blue Light Sensing and Reporting System

This part was designed to build the complete blue light sensing and reporting system in a single plasmid. Promoter BBa_J23117 is a constitutive promoter and not a strong one. It will keep a certain concentration of LovTAP in bacteria. When exposure to blue light, the absorption of a photon will leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal SpeI site found at 918
Illegal SpeI site found at 926
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 918
Illegal SpeI site found at 926
21
COMPATIBLE WITH RFC[21]
23
INCOMPATIBLE WITH RFC[23]
Illegal SpeI site found at 918
Illegal SpeI site found at 926
25
INCOMPATIBLE WITH RFC[25]
Illegal SpeI site found at 918
Illegal SpeI site found at 926
Illegal AgeI site found at 1525
Illegal AgeI site found at 1637
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 595

Functional Parameters

n/a	Blue Light Sensor+PtrpL+mRFP1

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K560000 short</partinfo>
+== Blue Light Sensing and Reporting System ==
+This part was designed to build the complete blue light sensing and reporting system in a single plasmid. Promoter BBa_J23117 is a constitutive promoter and not a strong one. It will keep a certain concentration of LovTAP in bacteria. When exposure to blue light, the absorption of a photon will leads to conformational rearrangements in LovTAP. The LovTAP can then bind to DNA and repress the transcription of genes following the trpL promoter. Here we use RFP as the reporter.
+[[Image:Whu-Part1.png]]