Difference between revisions of "Part:BBa K646000:Design"
(→Design Notes) |
|||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K646000 short</partinfo> | <partinfo>BBa_K646000 short</partinfo> | ||
Line 7: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | The gene was digested with | + | The gene was digested with XbaI and SpeI for the BioBrick construction due to the primer design, and the gene orientation was screened by an XbaI digest of the constructed BioBrick. For subsequent usage of this part, iGEM BioBrick assembly protocol could be used; digest using either EcoRI with SpeI or XbaI with PstI. |
− | + | ||
− | + | ||
===Source=== | ===Source=== |
Latest revision as of 12:47, 30 September 2011
Hemeoxygenase with RBS
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The gene was digested with XbaI and SpeI for the BioBrick construction due to the primer design, and the gene orientation was screened by an XbaI digest of the constructed BioBrick. For subsequent usage of this part, iGEM BioBrick assembly protocol could be used; digest using either EcoRI with SpeI or XbaI with PstI.
Source
Http://www.ncbi.nlm.nih.gov/gene/953483