Difference between revisions of "Part:BBa K625002"
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==References==
 
==References==
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<span id="Ref1">[1] [http://aem.asm.org/cgi/content/abstract/64/2/748 S. Panke, J. M. Sanchez Romero, V. de Lorenzo: '''Engineering of Quasi-Natural ''Pseudomonas putida'' Strains for Toluene Metabolism through an ortho-Cleavage Degradation Pathway ''', Applied and Environmental Microbiology, 1998, Vol. 64, No. 2]</span>

Revision as of 10:18, 30 September 2011

Pu promoter long version with stop codon

This is an adapted version of the Pu promoter BBa_I723020. The original design contains the RBS and first 81bp of the XylU. To prevent unwanted fusion proteins from emerging (when this BioBrick is cloned without scar), a double stop codon was inserted at the end of the promoter.

Characterization

Experimental setup

E. coli strain JM101 was transformed with two plasmids containing the transcriptional regulator XylR, the degradation cassette xylMABN and a GFP reporter coupled to BBa_K625002 respectively BBa_K625003. For the first two of those we used the plasmid pCK04AxylR according to [1].




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 195
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

[1] [http://aem.asm.org/cgi/content/abstract/64/2/748 S. Panke, J. M. Sanchez Romero, V. de Lorenzo: Engineering of Quasi-Natural Pseudomonas putida Strains for Toluene Metabolism through an ortho-Cleavage Degradation Pathway , Applied and Environmental Microbiology, 1998, Vol. 64, No. 2]