Difference between revisions of "Part:BBa K660200:Design"
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===Design Notes=== | ===Design Notes=== | ||
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===Source=== | ===Source=== | ||
Latest revision as of 11:58, 29 September 2011
Latherin (Surfactant Protein)
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 200
Illegal PstI site found at 431
Illegal PstI site found at 569 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 200
Illegal PstI site found at 431
Illegal PstI site found at 569 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 514
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 200
Illegal PstI site found at 431
Illegal PstI site found at 569 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 200
Illegal PstI site found at 431
Illegal PstI site found at 569 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
Latherin was originally isolated from horse sweat. It was expressed in E.coli by researchers at University of Glasgow. We received it from them in a plasmid. We then amplified it up by PCR to add biobrick prefix and suffix.