Difference between revisions of "Part:BBa K672000:Design"
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===Design Notes===
 
===Design Notes===
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We wanted to make sure that both parts worked in our specific conditions, and for that we did tests in the lab with good results.  
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We wanted to make sure that both parts worked in our specific conditions, and for that we did tests in the lab as shown in the experience.  
  
  
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===Source===
 
===Source===
  
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Pyear + RBS + GFP + TT + TT + hyBb + ompA + AOX. It comes from the combination of two other existing Biobricks.
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It comes from the combination of two other existing Biobricks:<br>
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For more information consult both other parts:
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[[Part:BBa_K381001]] *Pyear + RBS + GFP + TT + TT<br>
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https://parts.igem.org/Part:BBa_K381001  
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[[Part:BBa_K410000]] *hyBb + ompA + AOX
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https://parts.igem.org/Part:BBa_K410000
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===References===
 
===References===
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== Pyear promoter ==
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*Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of ''yeaR-yoaG'' operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in ''Escherichia col''i K-12.'' J. Bacteriol.'' '''189''', 7539-7548
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== GFP ==
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*See [[Part:BBa_E0040:Design#References]].
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== Aox ==
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*Grant, N et al. “Two Cys or Not Two Cys? That is the Question; Alternative Oxidase in the Thermogenic Plant sacred Lotus.” Plant Physiology 150 (2009): 987-995. {PAPER}
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*HybB Sequence:[[https://parts.igem.org/Part:BBa_J43001:Design]]
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*OmpA Sequence:[[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Datasheet/e8283dat.Par.0001.File.tmp/e8283dat.pdf]]
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*AoxA Sequence:[[http://www.ncbi.nlm.nih.gov/nuccore/226897260#sequence_226897260]]

Latest revision as of 07:37, 29 September 2011

Nitrate sensing at low temperatures.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 394
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 2298


Design Notes

We wanted to make sure that both parts worked in our specific conditions, and for that we did tests in the lab as shown in the experience.


Source

It comes from the combination of two other existing Biobricks:
Part:BBa_K381001 *Pyear + RBS + GFP + TT + TT
Part:BBa_K410000 *hyBb + ompA + AOX

References

Pyear promoter

  • Lin, H.-Y., Bledsoe, P.J., and Stewart, V. 2007. Activation of yeaR-yoaG operon transcription by the nitrate-responsive regulator NarL is independent of oxygen-responsive regulator Fnr in Escherichia coli K-12. J. Bacteriol. 189, 7539-7548

GFP

Aox