Difference between revisions of "Part:BBa K649301"

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Ptrc and RBS are from pTrc99A.<br />
 
Ptrc and RBS are from pTrc99A.<br />
  
This part was created for assay.
+
You should NOT ligate this part into high-copy vector.<br />
 +
 
 +
In our test, ligated into pSB1C3, <span style="font-style:italic;">E.coli</span> dosen't glow after 24 hours incubation.<br />
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 06:13, 29 September 2011

Ptrc-RBS-rocF

rocF is the same as BBa_K649300.
Ptrc and RBS are from pTrc99A.

You should NOT ligate this part into high-copy vector.

In our test, ligated into pSB1C3, E.coli dosen't glow after 24 hours incubation.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 230
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]