Difference between revisions of "Part:BBa K672000:Experience"
(→‎Experiments with BBa_K672000)
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# The best growth is obtained in Luria Broth. <br>
 
# The best growth is obtained in Luria Broth. <br>
 
# Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.
 
# Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.
 +
 +
===Experiments with Other BioBricks===
 +
 +
BBa_K381001 Bristol
 +
 +
Sample ID         Temperature (°C) Substrate Absorbance @420 nm
 +
BBa_K381001 Bristol        23                   LB               0.599
 +
BBa_K381001 Bristol     37                   LB               0.961
 +
BBa_K381001 Bristol     8                   LB               0.015
 +
BBa_K381001 Bristol     23                    MM               0.341
 +
BBa_K381001 Bristol     37                   MM               0.351
 +
BBa_K381001 Bristol     8                   MM               0.021
 +
BBa_K381001 Bristol     23                   SS               0.010
 +
BBa_K381001 Bristol     37                   SS               0.006
 +
BBa_K381001 Bristol     8                   SS               0.005
 +
 +
From these data we could conclude that:
 +
• The BB grows best in LB.
 +
• It grows less in Minimal Media.
 +
• It shows almost no growth in Saline Solution.
 +
• Il grows best @37°C
 +
• It grows good @23°C
 +
• It shows almost no growth @8°C
 +
 +
 +
 +
BBa_K410000 GaTech
 +
 +
Sample ID     Temperature (°C) Substrate Absorbance @420 nm
 +
BBa_K410000 GaTech   23             LB                0.258
 +
BBa_K410000 GaTech   37             LB              0.624
 +
BBa_K410000 GaTech   8             LB               0.006
 +
BBa_K410000 GaTech   23             MM               0.275
 +
BBa_K410000 GaTech   37             MM               0.028
 +
BBa_K410000 GaTech   8             MM               0.004
 +
BBa_K410000 GaTech   23             SS               0.015
 +
BBa_K410000 GaTech   37             SS             -0.001
 +
BBa_K410000 GaTech   8             SS               0.014
 +
       
 +
From these data we could conclude that:
 +
• The BB grows best in LB.
 +
• It grows well in Minimal Media.
 +
• It shows almost no growth in Saline Solution.
 +
• Il grows best @37°C
 +
• It grows well @23°C (best in Minimal Media than LB)
 +
• It shows almost no growth @8°C
  
 
===User Reviews===
 
===User Reviews===

Revision as of 05:24, 29 September 2011

Experiments with BBa_K672000

We did Spectrophotometry measurments, obtaining the following results:

Renboresults.jpg

Which leads us to the following conclusions:

  1. The best growth is obtained in Luria Broth.
  2. Even though we obtained some growth at 23°C in the LB substrate, the Biobrick doesn't fulfill our expectations and requirements.

Experiments with Other BioBricks

BBa_K381001 Bristol

Sample ID Temperature (°C) Substrate Absorbance @420 nm BBa_K381001 Bristol 23 LB 0.599 BBa_K381001 Bristol 37 LB 0.961 BBa_K381001 Bristol 8 LB 0.015 BBa_K381001 Bristol 23 MM 0.341 BBa_K381001 Bristol 37 MM 0.351 BBa_K381001 Bristol 8 MM 0.021 BBa_K381001 Bristol 23 SS 0.010 BBa_K381001 Bristol 37 SS 0.006 BBa_K381001 Bristol 8 SS 0.005

From these data we could conclude that: • The BB grows best in LB. • It grows less in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows good @23°C • It shows almost no growth @8°C


BBa_K410000 GaTech

Sample ID Temperature (°C) Substrate Absorbance @420 nm BBa_K410000 GaTech 23 LB 0.258 BBa_K410000 GaTech 37 LB 0.624 BBa_K410000 GaTech 8 LB 0.006 BBa_K410000 GaTech 23 MM 0.275 BBa_K410000 GaTech 37 MM 0.028 BBa_K410000 GaTech 8 MM 0.004 BBa_K410000 GaTech 23 SS 0.015 BBa_K410000 GaTech 37 SS -0.001 BBa_K410000 GaTech 8 SS 0.014

From these data we could conclude that: • The BB grows best in LB. • It grows well in Minimal Media. • It shows almost no growth in Saline Solution. • Il grows best @37°C • It grows well @23°C (best in Minimal Media than LB) • It shows almost no growth @8°C

User Reviews

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