Difference between revisions of "Part:BBa K619889:Design"

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===Design Notes===
 
===Design Notes===
  
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The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.  This caused a G to A mutation in the Shine-Delgarno which, in theory, would not affect the hairpin since this is an unpaired region of the hairpin.  We assume this is a mistake from the primers ordered, however, it still behaves as it should.  We have not compared expression levels with the wildtype Shine-Delgarno.
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The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.  We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA.  This should not have a drastic affect the hairpin structure since it is in an unpaired region.
  
 
===Source===
 
===Source===
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===References===
 
===References===
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[[Johansson J. et al., An RNA Thermosensor Controls Expression of Virulence Genes in Listeria Monocytogenes. (2001)  Cell Vol. 110, 551-561.]]

Latest revision as of 04:22, 29 September 2011

prfA-UTR, RNA thermosensor from Listeria monocytogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region.

Source

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.

References

Johansson J. et al., An RNA Thermosensor Controls Expression of Virulence Genes in Listeria Monocytogenes. (2001) Cell Vol. 110, 551-561.