Difference between revisions of "Part:BBa K619889:Design"
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<partinfo>BBa_K619889 short</partinfo> | <partinfo>BBa_K619889 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
| − | + | The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region. | |
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
| + | [[Johansson J. et al., An RNA Thermosensor Controls Expression of Virulence Genes in Listeria Monocytogenes. (2001) Cell Vol. 110, 551-561.]] | ||
Latest revision as of 04:22, 29 September 2011
prfA-UTR, RNA thermosensor from Listeria monocytogenes
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 18
- 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 18
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 18
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 18
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region.
Source
The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.