Difference between revisions of "Part:BBa K619889:Design"

(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
  
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The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.  This caused a G to A mutation in the Shine-Delgarno which, in theory, would not affect the hairpin since this is an unpaired region of the hairpin.  We assume this is a mistake from the primers ordered, however, it still behaves as it should.  We have not compared expression levels with the wildtype Shine-Delgarno.
+
The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.  We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA.  This should not have a drastic affect the hairpin structure since it is in an unpaired region.
  
 
===Source===
 
===Source===

Revision as of 04:18, 29 September 2011

prfA-UTR, RNA thermosensor from Listeria monocytogenes


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 18
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 18
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 18
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 18
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them. We optimized the Shine-Delgarno for E.coli by mutating the GGGGGA to GGAGGA. This should not have a drastic affect the hairpin structure since it is in an unpaired region.

Source

The source of the DNA sequence is Listeria monocytogenes prfA-UTR, however we constructed this part by simply ordering DNA oligos and annealing them.

References