Difference between revisions of "Part:BBa K511300"

 
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K511300 short</partinfo>
 
<partinfo>BBa_K511300 short</partinfo>
  
 
This part encodes a major mammalian cell-signaling protein, the Notch-1 receptor, engineered to replace the original 12xCSL-binding transactvation domain with a minimal version of the Gal4 transactivator (Gal4-ESN). In the presence of the Delta ligand protein from another cell, this transactivation domain is cleaved and released to the Notch producing cell's nucleus. In contrast, if Delta is present on the Notch producing cell, the Delta protein will cis-inhibit the Notch receptor, desensitizing the receptor to activation from external Delta molecules. This receptor is singularly useful for cell patterning, lineage decision-making, and other forms of signaling-intensive processes.
 
This part encodes a major mammalian cell-signaling protein, the Notch-1 receptor, engineered to replace the original 12xCSL-binding transactvation domain with a minimal version of the Gal4 transactivator (Gal4-ESN). In the presence of the Delta ligand protein from another cell, this transactivation domain is cleaved and released to the Notch producing cell's nucleus. In contrast, if Delta is present on the Notch producing cell, the Delta protein will cis-inhibit the Notch receptor, desensitizing the receptor to activation from external Delta molecules. This receptor is singularly useful for cell patterning, lineage decision-making, and other forms of signaling-intensive processes.
 +
 +
For more information, consult the MIT iGEM 2011 wiki <html><a href="http://2011.igem.org/Team:MIT/Results">here.</a></html>
 +
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:57, 29 September 2011

Notch-Gal4-ESN Juxtacrine Signaling Receptor MammoBlock

This part encodes a major mammalian cell-signaling protein, the Notch-1 receptor, engineered to replace the original 12xCSL-binding transactvation domain with a minimal version of the Gal4 transactivator (Gal4-ESN). In the presence of the Delta ligand protein from another cell, this transactivation domain is cleaved and released to the Notch producing cell's nucleus. In contrast, if Delta is present on the Notch producing cell, the Delta protein will cis-inhibit the Notch receptor, desensitizing the receptor to activation from external Delta molecules. This receptor is singularly useful for cell patterning, lineage decision-making, and other forms of signaling-intensive processes.

For more information, consult the MIT iGEM 2011 wiki here.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
    Illegal NotI site found at 5216
    Illegal NotI site found at 5224
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 2925
    Illegal XhoI site found at 5507
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 250
    Illegal PstI site found at 655
    Illegal PstI site found at 985
    Illegal PstI site found at 1216
    Illegal PstI site found at 1313
    Illegal PstI site found at 2503
    Illegal PstI site found at 4381
    Illegal NgoMIV site found at 516
    Illegal NgoMIV site found at 2628
    Illegal NgoMIV site found at 2684
    Illegal NgoMIV site found at 2727
    Illegal NgoMIV site found at 3512
    Illegal NgoMIV site found at 4529
    Illegal NgoMIV site found at 4706
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 5426
    Illegal BsaI.rc site found at 85
    Illegal BsaI.rc site found at 1793
    Illegal BsaI.rc site found at 2708
    Illegal BsaI.rc site found at 5165