Difference between revisions of "Part:BBa K511201"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K511201 short</partinfo> | <partinfo>BBa_K511201 short</partinfo> | ||
This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants. | This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants. | ||
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+ | For more information, consult the MIT iGEM 2011 wiki <html><a href="http://2011.igem.org/Team:MIT/Results">here.</a></html> | ||
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<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here |
Revision as of 03:56, 29 September 2011
Mnt-VP16 Transactivator MammoBlock
This part encodes a protein that combines the Mnt DNA-binding domain with the VP16 viral transactivation domain. This synthetic transactivator is able to moderately activate expression from minCMV-Mnt promoter variants.
For more information, consult the MIT iGEM 2011 wiki here.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 93
Illegal PstI site found at 106 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 93
Illegal PstI site found at 106 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 93
- 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 93
Illegal PstI site found at 106 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 93
Illegal PstI site found at 106 - 1000COMPATIBLE WITH RFC[1000]