Difference between revisions of "Part:BBa K511000"
| Line 5: | Line 5: | ||
Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription. | Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription. | ||
| − | + | <html> | |
<img src='https://static.igem.org/mediawiki/2011/thumb/0/0c/Doxiptg2inputandgate.jpg/800px-Doxiptg2inputandgate.jpg' style="width:60%"> | <img src='https://static.igem.org/mediawiki/2011/thumb/0/0c/Doxiptg2inputandgate.jpg/800px-Doxiptg2inputandgate.jpg' style="width:60%"> | ||
Revision as of 03:53, 29 September 2011
Hef1a-LacOid Promoter MammoBlock
This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.
Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.
To see further information about this part, consult the Results found on the iGEM 2011 wiki.
Sequence and Features