Difference between revisions of "Part:BBa K511000"
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Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.  
 
Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.  
  
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To see further information about this part, consult the Results found [http://2011.igem.org/Team:MIT/Results/#cmv-to]here on the iGEM 2011 wiki.
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To see further information about this part, consult the Results found [[http://2011.igem.org/Team:MIT/Results/#cmv-to|here]] on the iGEM 2011 wiki.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 03:48, 29 September 2011

Hef1a-LacOid Promoter MammoBlock

This part encodes a promoter with low-level, constitutive activity that can be repressed by variants of the LacI transcriptional repressor.

Cotransfection of a construct containing a yellow fluorescent protein (EYFP) driven by the Hef1a-LacO promoter with a construct containing a LacI-KRAB transcriptional repressor driven by the TRE-Tight promoter proves that it is possible to repress Hef1a-LacO expression with the LacI-KRAB LacI variant, which includes a Kruppel-associated box motif previously shown to silence chromatin and thereby inhibit transcription.

To see further information about this part, consult the Results found here on the iGEM 2011 wiki.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 292
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 670
    Illegal BamHI site found at 255
    Illegal XhoI site found at 1069
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 416
    Illegal PstI site found at 921
    Illegal NgoMIV site found at 804
    Illegal AgeI site found at 80
  • 1000
    COMPATIBLE WITH RFC[1000]