Difference between revisions of "Part:BBa K542007"
 
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Made by assembling [https://parts.igem.org/Part:BBa_P0440 BBa_P0440] with [https://parts.igem.org/Part:BBa_K331033 BBa_K331033].
 
Made by assembling [https://parts.igem.org/Part:BBa_P0440 BBa_P0440] with [https://parts.igem.org/Part:BBa_K331033 BBa_K331033].
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The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.
 
The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.
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Intermediate part to assemble the "Co-localization Testing Construct".
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Intermediate part to assemble the "Co-localization Testing Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
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This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
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[[image:uoflECdatasheet.png|150px]]
  
 
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Latest revision as of 02:00, 29 September 2011

Unregulated TetR with pTet Regulated Arg-tagged ECFP

Made by assembling BBa_P0440 with BBa_K331033.

The pTet promoter is constitutively "on"; thus, Arg-tagged ECFP will be produced constitutively. By placing an inducible promoter upstream of this part, expression of the Arg-tagged ECFP will be regulated inversely. If the pLacI promoter (BBa_R0010) is placed upstream, the presence of IPTG will cease Arg-tagged ECFP expression.

Intermediate part to assemble the "Co-localization Testing Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]