Difference between revisions of "Part:BBa K542004"
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Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. <br> | Note that the pLacI promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0010 BBa_R0010]) is regulating the Lumazine Synthase gene, not the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible. <br> | ||
| − | The microcompartment portion to the "Co-localization Construct". | + | The microcompartment portion to the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008]) |
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| − | This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha | + | This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below. |
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[[image:uoflECdatasheet.png|150px]] | [[image:uoflECdatasheet.png|150px]] | ||
Latest revision as of 01:59, 29 September 2011
pLacI Regulated Lumazine Synthase with Transciptional Terminator and pBAD
Made by assembling BBa_K542001 with BBa_K542003.
Note that the pLacI promoter (BBa_R0010) is regulating the Lumazine Synthase gene, not the pBAD promoter (BBa_I13453) which is downstream. Therefore, Lumazine Synthase production is IPTG inducible.
The microcompartment portion to the "Co-localization Construct". (BBa_K542008)
This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 961
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 901
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]