Difference between revisions of "Part:BBa K542003"

Latest revision as of 01:59, 29 September 2011

pBAD Regulated TetR

Made by assembling BBa_I13453 with BBa_P0440.

Production of TetR is regulated by the pBAD promoter (BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")

Intermediate part to assemble the "Co-localization Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 125
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 65
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [https://parts.igem.org/Part:BBa_P0440 BBa_P0440].
+<br><br>
 Production of TetR is regulated by the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[https://parts.igem.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")
 <br><br>
-Intermediate part to assemble the "Co-localization Construct".
+Intermediate part to assemble the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
 <br><br>
-Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct.
+This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
 <br><br>
 [[image:uoflECdatasheet.png|150px]]