Difference between revisions of "Part:BBa K542003"
 
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Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [https://parts.igem.org/Part:BBa_P0440 BBa_P0440].
 
Made by assembling [https://parts.igem.org/wiki/index.php?title=Part:BBa_I13453 BBa_I13453] with [https://parts.igem.org/Part:BBa_P0440 BBa_P0440].
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Production of TetR is regulated by the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[https://parts.igem.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")<br>
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Production of TetR is regulated by the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]). TetR is an inhibitor of the constitutively "on" promoter pTet--[https://parts.igem.org/wiki/index.php?title=Part:BBa_R0040 BBa_R0040]). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")
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Intermediate part to assemble the "Co-localization Construct".  
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Intermediate part to assemble the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
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This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
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[[image:uoflECdatasheet.png|150px]]
  
 
<!-- Add more about the biology of this part here
 
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Latest revision as of 01:59, 29 September 2011

pBAD Regulated TetR

Made by assembling BBa_I13453 with BBa_P0440.

Production of TetR is regulated by the pBAD promoter (BBa_I13453). TetR is an inhibitor of the constitutively "on" promoter pTet--BBa_R0040). Therefore, this part may be used in conjunction with the pTet promoter as an "inverter"; pTet will be "turned off" in the presence of arabinose. (ie. arabinose -> pBAD "on" -> TetR -> pTet "off")

Intermediate part to assemble the "Co-localization Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 125
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 65
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]