Difference between revisions of "Part:BBa K542002"

 
 
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<partinfo>BBa_K542002 short</partinfo>
 
<partinfo>BBa_K542002 short</partinfo>
  
 
Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453].
 
Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453].
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Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible). <br><br>
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Intermediate part to assemble the "Co-localization Construct". ([https://parts.igem.org/Part:BBa_K542008 BBa_K542008])
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This part was characterized in <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.
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[[image:uoflECdatasheet.png|150px]]
  
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Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. not arabinose inducible).
 
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Intermediate part to assemble the "Co-localization Construct".
 
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Latest revision as of 01:58, 29 September 2011

Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)

Made by assembling BBa_K542000 with BBa_I13453.

Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).

Intermediate part to assemble the "Co-localization Construct". (BBa_K542008)

This part was characterized in BBa_K542008 in E. coli strain DH5alpha to demonstrate its functionality as an intermediate in that construct. The data sheet for BBa_K542008 is shown below.

UoflECdatasheet.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 735
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 675
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]