Difference between revisions of "Part:BBa K569010:Design"
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foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' | foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' | ||
reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' | reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' | ||
− | CheZ | + | CheZ*(mutant) |
5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' | 5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' | ||
5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3' | 5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3' |
Latest revision as of 01:08, 29 September 2011
ctx+CheZ mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 818
Design Notes
We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) from an old backbone to add the BioBrick restriction enzymes sites.
CTX foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' CheZ*(mutant) 5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' 5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'
Source
CheZ*(mutant) --Kindly given by Dr. Ruth Silversmith from the University of North Carolina School of Medicine.
J07011(MIT)