Difference between revisions of "Part:BBa K569010:Design"
(→Design Notes) |
(→Design Notes) |
||
(2 intermediate revisions by the same user not shown) | |||
Line 6: | Line 6: | ||
===Design Notes=== | ===Design Notes=== | ||
− | We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) from an old backbone | + | We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) |
+ | from an old backbone to add the BioBrick restriction enzymes sites. | ||
+ | |||
Line 12: | Line 14: | ||
foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' | foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' | ||
reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' | reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' | ||
− | CheZ | + | CheZ*(mutant) |
5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' | 5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' | ||
− | 5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3' | + | 5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3' |
− | + | ||
===Source=== | ===Source=== | ||
− | + | CheZ*(mutant) --Kindly given by Dr. Ruth Silversmith from the University of North Carolina School of Medicine. | |
+ | |||
+ | J07011(MIT) | ||
===References=== | ===References=== |
Latest revision as of 01:08, 29 September 2011
ctx+CheZ mutant
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 818
Design Notes
We designed and used primers to PCR out both the CTX from CTX-GFP and CheZ*(mutant) from an old backbone to add the BioBrick restriction enzymes sites.
CTX foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' CheZ*(mutant) 5'-CCATCTAGAATGCAACCATCAATCAAACCTG-3' 5'-CCAACTAGTCCACATCAGGCAATACAAATCA-3'
Source
CheZ*(mutant) --Kindly given by Dr. Ruth Silversmith from the University of North Carolina School of Medicine.
J07011(MIT)