Difference between revisions of "Part:BBa K648034:Experience"

Latest revision as of 00:59, 29 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K648034

User Reviews

We used this part to test that the promoter on the right side of Or was working properly. The production of red fluorescence protein was measured using a TECAN machine, and compared to a normal strand of E. coli. The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional. A graph of the results is shown below.

UNIQ71fcab0428158c84-partinfo-00000000-QINU UNIQ71fcab0428158c84-partinfo-00000001-QINU

@@ Line 1: / Line 1: @@
 __NOTOC__
 This experience page is provided so that any user may enter their experience using this part.<BR>Please enter
@@ Line 7: / Line 6: @@
 ===User Reviews===
+We used this part to test that the promoter on the right side of Or was working properly. The production of red fluorescence protein was measured using a TECAN machine, and compared to a normal strand of E. coli.  The data collected while the samples were in exponential growth was used to determine a rate of expression of the rfp. Compared to the control, the rate of fluorescence was much higher in our test subject, about 4227 compared to 418 (fu/OD600nm). This suggests that the promoter on Or is functional.  A graph of the results is shown below.
+[[image:PSUOrTest.png|800px]]
+[[Image:OrTest2.jpg|800px]]
 <!-- DON'T DELETE --><partinfo>BBa_K648034 StartReviews</partinfo>
 <!-- Template for a user review