Difference between revisions of "Part:BBa K569006:Design"
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===Source=== | ===Source=== | ||
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===References=== | ===References=== |
Latest revision as of 00:56, 29 September 2011
ctx+CheY
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 428
- 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 428
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We designed and used primers to PCR out both the CTX from CTX-GFP and Chey from an old backbone
CTX foward-5'-GTA TCA CGA GGC AGA ATT TCA -3' reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3' CheY foward-5'-ccatctagacacctgcaaaaggagatgg-3' revers-5'-ccaactagtcacctgcaaaaagggacat-3'
Source
J07011 (MIT)
K422007 (Basel)