Difference between revisions of "Part:BBa K566029"

Latest revision as of 00:30, 29 September 2011

CcaR gene (constitutive expression)

CcaR is the response regulator of CcaS, wich is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with the absorption of red light.

Sequence and Features

Assembly Compatibility:

10
INCOMPATIBLE WITH RFC[10]
Illegal EcoRI site found at 69
Illegal SpeI site found at 765
12
INCOMPATIBLE WITH RFC[12]
Illegal EcoRI site found at 69
Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal SpeI site found at 765
21
INCOMPATIBLE WITH RFC[21]
Illegal EcoRI site found at 69
Illegal BamHI site found at 466
23
INCOMPATIBLE WITH RFC[23]
Illegal EcoRI site found at 69
Illegal SpeI site found at 765
25
INCOMPATIBLE WITH RFC[25]
Illegal EcoRI site found at 69
Illegal SpeI site found at 765
1000
COMPATIBLE WITH RFC[1000]

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 __NOTOC__
 <partinfo>BBa_K566029 short</partinfo>
-CcaR gene (constitutive expression)
+CcaR is the response regulator of CcaS, wich is a member of cyanobacteriochrome family of proteins. Phycocyanobillin (PCB) binds at a conserved cysteine domain of CcaS and activates signaling activity. CcaS, along CcaR, activates transcription of promoter pCpcG2. Activation shows max response with 535 nm wavelength green light and with 672 nm wavelength red light. When absorbing green light, CcaS autophosphorylates and then carries out phosphotransfer to CcaR. Phosphorylated CcaR (CcaR*) acts as a transcription factor for pCpcG2. This process can be reverted with the absorption of red light.
 <!-- Add more about the biology of this part here