Difference between revisions of "Part:BBa K569014:Design"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K569014 short</partinfo> | <partinfo>BBa_K569014 short</partinfo> | ||
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===Design Notes=== | ===Design Notes=== | ||
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+ | '''FGFR''' | ||
+ | - 1. Added restriction sites XbaI and SpeI via PCR mechanism | ||
+ | - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene | ||
+ | ===Source=== | ||
− | + | ToxR-- J07009 (MIT) | |
− | + | FGFR-- Received from Openbiosystems (Receptor 1: isoform 15) | |
+ | |||
+ | Strong Constitutive Promoter-- BBa_J23119 (Berkeley) | ||
===References=== | ===References=== |
Latest revision as of 00:14, 29 September 2011
SCP+ToxR+FGFR
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1266
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 844
Illegal NgoMIV site found at 1435 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
FGFR
- 1. Added restriction sites XbaI and SpeI via PCR mechanism - 2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
Source
ToxR-- J07009 (MIT)
FGFR-- Received from Openbiosystems (Receptor 1: isoform 15)
Strong Constitutive Promoter-- BBa_J23119 (Berkeley)