Difference between revisions of "Part:BBa K569013:Design"

 
(→‎Source)
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
−
 
 
__NOTOC__
 
__NOTOC__
 
<partinfo>BBa_K569013 short</partinfo>
 
<partinfo>BBa_K569013 short</partinfo>
Line 7: Line 6:
  
 
===Design Notes===
 
===Design Notes===
−
/
 
  
 +
'''FGFR'''
 +
      1. Added restriction sites XbaI and SpeI via PCR mechanism
 +
      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene
  
 +
===Source===
  
−
===Source===
+
ToxR-- J07009 (MIT)
  
−
?
+
FGFR-- Received from Openbiosystems
 +
 +
PyeaR-- BBa_K216005 (Edinburgh)
  
 
===References===
 
===References===

Latest revision as of 00:12, 29 September 2011

PyeaR+ToxR+FGFR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1331
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 909
    Illegal NgoMIV site found at 1500
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

FGFR 

      1. Added restriction sites XbaI and SpeI via PCR mechanism
      2. Did a point mutation experiment because there was an EcoRI restriction site within the gene

Source

ToxR-- J07009 (MIT)

FGFR-- Received from Openbiosystems

PyeaR-- BBa_K216005 (Edinburgh)

References