Difference between revisions of "Part:BBa K590017"

Revision as of 23:14, 28 September 2011

mamHIEJKL in vector pGA3K3

This part is made by [http://2011.igem.org/Team:Washington UW iGEM Team 2011]. It includes 6 genes mamHI, mamE, mamJ, mamKL as well as the native mamAB promoter, all in a pGA3k3 plasmid backbone. All the genes were found essential for magnetosome formation in Magnetospirillum magneticum strain AMB-1.

Usage and Biology

We made this construct from 2 kilobase gene extractions from AMB-1. These fragments were assembled using the [http://2010.igem.org/Team:Cambridge/Gibson/RFC Gibson cloning] method instead of standard assembly. This super-assembly is known as "Part one" of the full 16kb mamAB reassembly project. This part is in a medium copy backbone and it belongs to the [http://2011.igem.org/Team:Washington/Magnetosomes/Magnet_Toolkit Magnetosome Toolkit].

The gel image on the left is HIEJKL(~9000bp), we extracted this band and "gibsoned" it with pGA3k3 plasmid backbone(~3000bp), the right image.

1kb Ladder (left), mamHIEJKL (right)

1kb Ladder (left), 3k3 backbone (right)

After Gibson cloning and transformation, cell chain formation was observed in microscopy(below) when this plasmid was transformed in E.coli.

Sequence and Features