Difference between revisions of "Part:BBa K566012"

 
 
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<partinfo>BBa_K566012 short</partinfo>
 
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cI repressor from Lambda phage optimized for E. coli cI (+LVA).
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cI repressor from Lambda phage optimized for ''E. coli''. Includes LVA tag.
  
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Preferential codons for better expression into E. coli.
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Preferential codons to improve their expression in ''E. coli''.
  
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Includes LVA tag.
 
  
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<!-- Add more about the biology of this part here
 
 
===Usage and Biology===
 
===Usage and Biology===
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The lambda repressor is a dimeric protein also known as the cI protein. Lambda cI protein has the capability to bind several operators within a complex regulatory region that involves mainly three promoters (pRM, pR and pL). There are two well-known sets of cI binding sites in lambda (OR and OL) spaced 2.4 kb apart and composed of three operators each (OR1:OR2:OR3; OL1:OL2:OL3) (figure 1). cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein.
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[[Image:CI.png|thumb|center|600px|Figure 1. The λ operators OL and OR. The promoter pRM transcribes cI, rexA, and rexB in a lysogen, which sum up to size of ∼2.4 Kb.
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Figure taken and modified from Court DL et al. (2007) <i>J Bacteriol</i> 189:298–304.
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.]]
  
 
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Latest revision as of 22:49, 28 September 2011

cI repressor from Lambda phage optimized for E. coli cI (+LVA)

cI repressor from Lambda phage optimized for E. coli. Includes LVA tag.

Preferential codons to improve their expression in E. coli.


Usage and Biology

The lambda repressor is a dimeric protein also known as the cI protein. Lambda cI protein has the capability to bind several operators within a complex regulatory region that involves mainly three promoters (pRM, pR and pL). There are two well-known sets of cI binding sites in lambda (OR and OL) spaced 2.4 kb apart and composed of three operators each (OR1:OR2:OR3; OL1:OL2:OL3) (figure 1). cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM. Modified with a LVA tag for rapid degradation of the protein.


Figure 1. The λ operators OL and OR. The promoter pRM transcribes cI, rexA, and rexB in a lysogen, which sum up to size of ∼2.4 Kb. Figure taken and modified from Court DL et al. (2007) J Bacteriol 189:298–304. .

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 463
  • 1000
    COMPATIBLE WITH RFC[1000]