Difference between revisions of "Part:BBa K652004"
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This part contains an RBS, the type III Zoarces elongatus antifreeze protein (ZeAFP), followed by a terminator.
 
This part contains an RBS, the type III Zoarces elongatus antifreeze protein (ZeAFP), followed by a terminator.
  
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Note: Becuase this protein is so small (7kDa), expression may be difficult to detect on a Tris-Glycine gel. Using a Tris-Tricine system where small proteins are easier to see (not so diffuse) is advisable.
  
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<li> Note: Becuase this protein is so small (7kDa), expression may be difficult to detect on a Tris-Glycine gel. Using a Tris-Tricine system where small proteins are easier to see (not so diffuse) is advisable. </li>
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Revision as of 21:45, 28 September 2011

RBS-ZeAFP-Term

This part contains an RBS, the type III Zoarces elongatus antifreeze protein (ZeAFP), followed by a terminator.

Note: Becuase this protein is so small (7kDa), expression may be difficult to detect on a Tris-Glycine gel. Using a Tris-Tricine system where small proteins are easier to see (not so diffuse) is advisable.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 33
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 222
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]