Difference between revisions of "Part:BBa K652003"
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<partinfo>BBa_K652003 short</partinfo> | <partinfo>BBa_K652003 short</partinfo> | ||
This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Tenebrio molitor antifreeze protein (TmAFP) with a His tag at the N-terminus, followed by a terminator. Note, unlike BBa_K652002, this part does NOT contain a TEV cleavage site between eGFP and TmAFP | This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Tenebrio molitor antifreeze protein (TmAFP) with a His tag at the N-terminus, followed by a terminator. Note, unlike BBa_K652002, this part does NOT contain a TEV cleavage site between eGFP and TmAFP | ||
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| + | More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
Latest revision as of 21:41, 28 September 2011
T7-RBS-eGFP-TmAFP-His-Term
This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Tenebrio molitor antifreeze protein (TmAFP) with a His tag at the N-terminus, followed by a terminator. Note, unlike BBa_K652002, this part does NOT contain a TEV cleavage site between eGFP and TmAFP
More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 864
Illegal AgeI site found at 903
Illegal AgeI site found at 975 - 1000COMPATIBLE WITH RFC[1000]