Difference between revisions of "Part:BBa K652000"
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This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus, followed by a terminator.
 
This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus, followed by a terminator.
  
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Detailed characterization of this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale
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More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale
 +
 
 +
Figure 1: Gel confirming
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<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 21:39, 28 September 2011

T7-RBS-eGFP-TEV-RiAFP-His-Term

This part contains a T7 strong promoter, RBS, our eGFP-fusion protein containing the Rhagium inquistor antifreeze protein (RiAFP) with a His tag at the C-terminus, followed by a terminator.

More information about this and other parts can be found on our wiki under the project section: http://2011.igem.org/Team:Yale

Figure 1: Gel confirming


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 894
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1104
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 74
    Illegal SapI.rc site found at 87