Difference between revisions of "Part:BBa K590027"

Latest revision as of 20:11, 28 September 2011

AAR-PSB1C3-High consititutive

This part consists of Acyl-ACP Reductase in PSB1C3 high consititutive vector. This was an important negative control in testing the PetroBrick - which consists of Acyl-ACP Reductase and another part from Synechococcus elongatus, Aldehyde Decarbonylase.

Usage and Biology

This part produces AAR, which catalyzes the firststep in the conversion of cellular fatty acyl-ACPs to alkanes. AAR reduces the fatty acyl-ACP to an aldehyde. To generate alkanes, this part is paired with ADC in the PetroBrick.

GC plot showing that alkanes are only produced when both ADC and AAR are expressed. The red peak corresponds to AAR alone - which generates large amounts of aldehyde, which are then reduced to the corresponding alcohol by cellular alcohol dehydrogenases.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 470
Illegal NheI site found at 493
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1584
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 126
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K590027 short</partinfo>
-Acyl-ACP Reductase in PSB1C3 high consititutive vector
+This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase] in PSB1C3 high consititutive vector.  This was an important negative control in testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick] - which consists of Acyl-ACP Reductase and another part from ''Synechococcus elongatus'', [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 Aldehyde Decarbonylase].
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
-<!-- -->
+[[Image:Fatty_Acid_Biosynthesis.jpg|right|frameless|300px]]
+This part produces AAR, which catalyzes the firststep in the conversion of cellular fatty acyl-ACPs to alkanes.  AAR reduces the fatty acyl-ACP to an aldehyde.  To generate alkanes, this part is paired with ADC in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick].
+[[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed.  The red peak corresponds to AAR alone - which generates large amounts of aldehyde, which are then reduced to the corresponding alcohol by cellular alcohol dehydrogenases.]]
+<br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/>
 <span class='h3bb'>Sequence and Features</span>
 <partinfo>BBa_K590027 SequenceAndFeatures</partinfo>