Difference between revisions of "Part:BBa K590027"
(4 intermediate revisions by 2 users not shown) | |||
Line 1: | Line 1: | ||
− | |||
__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K590027 short</partinfo> | <partinfo>BBa_K590027 short</partinfo> | ||
− | Acyl-ACP Reductase in PSB1C3 high consititutive vector | + | This part consists of [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590032 Acyl-ACP Reductase] in PSB1C3 high consititutive vector. This was an important negative control in testing [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick] - which consists of Acyl-ACP Reductase and another part from ''Synechococcus elongatus'', [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590027 Aldehyde Decarbonylase]. |
+ | |||
− | |||
===Usage and Biology=== | ===Usage and Biology=== | ||
− | + | [[Image:Fatty_Acid_Biosynthesis.jpg|right|frameless|300px]] | |
+ | This part produces AAR, which catalyzes the firststep in the conversion of cellular fatty acyl-ACPs to alkanes. AAR reduces the fatty acyl-ACP to an aldehyde. To generate alkanes, this part is paired with ADC in [https://parts.igem.org/wiki/index.php?title=Part:BBa_K590025 the PetroBrick]. | ||
+ | |||
+ | [[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed. The red peak corresponds to AAR alone - which generates large amounts of aldehyde, which are then reduced to the corresponding alcohol by cellular alcohol dehydrogenases.]] | ||
+ | <br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/><br/> | ||
+ | |||
+ | |||
<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K590027 SequenceAndFeatures</partinfo> | <partinfo>BBa_K590027 SequenceAndFeatures</partinfo> |
Latest revision as of 20:11, 28 September 2011
AAR-PSB1C3-High consititutive
This part consists of Acyl-ACP Reductase in PSB1C3 high consititutive vector. This was an important negative control in testing the PetroBrick - which consists of Acyl-ACP Reductase and another part from Synechococcus elongatus, Aldehyde Decarbonylase.
Usage and Biology
This part produces AAR, which catalyzes the firststep in the conversion of cellular fatty acyl-ACPs to alkanes. AAR reduces the fatty acyl-ACP to an aldehyde. To generate alkanes, this part is paired with ADC in the PetroBrick.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1584
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]