Difference between revisions of "Part:BBa K590026"
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===Usage and Biology=== | ===Usage and Biology=== | ||
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| + | [[Image:Fatty_Acid_Biosynthesis.jpg|right|frameless|300px]] | ||
| + | This part produces ADC, which is the second step in the conversion of cellular fatty acyl-ACPs to alkanes. ADC decarbonylates the aldehyde produced in the first step by Acyl-ACP Reductase. This part is best used in combination with some kind of reductase, to form alkanes from the aldehydes made by the reductase. | ||
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| + | [[Image:Washinton_AARADCGC-MS.png|left|400px|thumb|GC plot showing that alkanes are only produced when both ADC and AAR are expressed. The blue peak corresponds to ADC alone - which only generates alkanes when sufficient aldehyde (produced by a cellular reductase) is present.]] | ||
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Revision as of 20:06, 28 September 2011
ADC-PSB1C3-High consititutive
This part consists of Aldehyde Decarbonylase, from Synechococcus elongatus, in PSB1C3 high consititutive vector. This was an important negative control in testing the PetroBrick - which consists of Aldehyde Decarbonylase and another part from Synechococcus elongatus, Acyl-ACP Reductase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 470
Illegal NheI site found at 493 - 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 1254
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 126
- 1000COMPATIBLE WITH RFC[1000]