Difference between revisions of "Part:BBa I712019:Experience"

Revision as of 19:27, 28 September 2011

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_I712019

User Reviews

UNIQ4738a27f390164d4-partinfo-00000000-QINU

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theos

The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector (:BBa_J13002) with the addition of D-luciferin. Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.

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Catramp

The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and D-luciferin is added, and is capable of working with standard luciferase testing kits and luminometers.

This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier (Dual Luciferase Assay Component).

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UNIQ4738a27f390164d4-partinfo-00000003-QINU The Tianjin 2010 igem team confirmed that this part works well since we have used this part to construct our parts which separate the luciferase gene. Our sequencing result reveal that the quality of this part is ok. UNIQ4738a27f390164d4-partinfo-00000004-QINU

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 The Cambridge team confirmed that this part did emit light from E. coli when placed under an expression vector ([https://parts.igem.org/wiki/index.php?title=Part:BBa_J13002 :BBa_J13002]) with the addition of D-luciferin.  Despite the claim that the "part contains a Eukaryotic ribosome binding site and non-standard prefix and suffix" we were able to express it in E. coli after adding a prokaryotic RBS and promoter using standard BioBrick assembly.
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+{|width='80%' style='border:1px solid gray'
+|-
+|width='10%'|
+<partinfo>BBa_I712019 AddReview 5</partinfo>
+<I>Catramp</I>
+|width='60%' valign='top'|
+The Utah_State 2011 iGEM Team has confirmed that this part emits light when placed under control of a promoter and D-luciferin is added, and is capable of working with standard luciferase testing kits and luminometers.
+This part has been improved upon with the addition of Dual Luciferase Assay intermediates that make construction of promoter testing devices with this part much easier ([https://parts.igem.org/Part:BBa_K654033 Dual Luciferase Assay Component]).
 |};