Difference between revisions of "Part:BBa K542002"

Revision as of 18:50, 28 September 2011

Lumazine Synthase with Transciptional Terminator and pBAD (No Promoter)

Made by assembling BBa_K542000 with BBa_I13453.

Note that the pBAD promoter (BBa_I13453) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible).

Intermediate part to assemble the "Co-localization Construct".

Datasheet for the test construct Part BBa_K542008 in E. coli strain DH5alpha demonstrating this is a functional intermediate in that construct.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 735
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 675
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 Made by assembling [https://parts.igem.org/Part:BBa_K542000 BBa_K542000] with [https://parts.igem.org/Part:BBa_I13453 BBa_I13453].
+<br><br>
-Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible). <br>
+Note that the pBAD promoter ([https://parts.igem.org/Part:BBa_I13453 BBa_I13453]) is downstream from the Lumazine Synthase gene. Therefore, pBAD is not regulating Lumazine Synthase production (ie. Lumazine Synthase is not arabinose inducible). <br><br>
 Intermediate part to assemble the "Co-localization Construct".
+<br><br>
+Datasheet for the test construct <html><a href="https://parts.igem.org/Part:BBa_K542008"><font color="blue">Part BBa_K542008</font></a></html> in <i>E. coli</i> strain DH5alpha demonstrating this is a functional intermediate in that construct.
+<br><br>
+[[image:uoflECdatasheet.png|150px]]
 <!-- Add more about the biology of this part here