Difference between revisions of "Part:BBa K656010:Design"

(→‎Design Notes)
(→‎References)
Line 16: Line 16:
  
 
===References===
 
===References===
 +
 +
 +
"Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products"
 +
http://aem.asm.org/cgi/content/abstract/76/11/3462

Revision as of 18:50, 28 September 2011

Anabaena pSac Promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Anabaena separates the process of photosynthesis and nitrogen fixation spatially, into two cell types. Heterocysts are cells responsible for forming an anaerobic environment in which nitrogen fixation can occur. The more numerous cell type, vegetative cells, are photosynthetic and capable of producing excess sugars.

The promoter we have modeled our part after is present in the Photo-system I of Anabaena 7120, and therefore its activity is both constitutive and confined to vegetative cells. This was done to confine sucrose secretion to only vegetative cells, as heterocysts are not producing sucrose through photosynthesis.

Source

Anaebaena PCC 7120

References

"Engineering Cyanobacteria To Synthesize and Export Hydrophilic Products" http://aem.asm.org/cgi/content/abstract/76/11/3462