Difference between revisions of "Part:BBa K566004"

Revision as of 08:12, 28 September 2011

CFP protein generator without transcription terminator

Protein generator of cyan fluorescent protein without transcription terminator. It may be negatively regulated by Mnt repressor (BBa_C0072).

Usage and Biology

Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea victoria . It possesses bright fluorescence with excitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 39
Illegal XhoI site found at 1
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 107
1000
COMPATIBLE WITH RFC[1000]

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	===Usage and Biology===		===Usage and Biology===
−	Is a monomeric protein generated on the basis of GFP-like protein from jellyfish Aequorea ~~macrodactyla~~ . It possesses bright fluorescence with ~~exitation~~/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.	+	Is a monomeric protein generated on the basis of GFP-like protein from jellyfish <i>Aequorea victoria</i> . It possesses bright fluorescence with excitation/emission maxima at 434 and 477 nm, respectively. It has an extinction coefficient of 26,000 M-1 cm-1.