Difference between revisions of "Part:BBa K566013:Design"
Line 16: | Line 16: | ||
==References== | ==References== | ||
− | <b> 1.</b> Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell | + | <b> 1.</b> Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. <i>Cell Science at a Glance</i> <b>5</b>:837-838 |
Latest revision as of 08:10, 28 September 2011
RFP optimized for E. coli (+LVA)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 570
Illegal AgeI site found at 682 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence was design considering use of preferential codons, as well, a complete search looking for illegal sites for classic BioBricks was performed, they were found and deleted.
Source
Sequence was taken from BioBrick BBa_J06505, then optimized and synthesized.
References
1. Patterson G, Rich N Day and David P (2001) Fluorescent protein spectra. Cell Science at a Glance 5:837-838