Difference between revisions of "Part:BBa K566012"

Revision as of 07:42, 28 September 2011

cI repressor from Lambda phage optimized for E. coli cI (+LVA)

cI repressor from Lambda phage optimized for E. coli. Includes LVA tag.

Preferential codons to improve their expression in E. coli.

Usage and Biology

The lambda repressor is a dimeric protein also known as the cI protein. cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 463
1000
COMPATIBLE WITH RFC[1000]

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 Preferential codons to improve their expression in ''E. coli''.
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+The lambda repressor is a dimeric protein also known as the cI protein. cI has a higher affinity for OR1 and OR2 operators, where binding positively regulates the pRM promoter. On the other hand, when bound to OR3 cI represses the promoter; however, due to a low binding affinity, a higher concentration of the protein is required. Thus, cI can act as both an activator -at low concentrations- and a repressor -at high concentrations- on pRM.
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