Difference between revisions of "Part:BBa K566007"
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<partinfo>BBa_K566007 short</partinfo>
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<partinfo>BBa_K566010 short</partinfo>
  
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PenI repressor from Bacillus licheniformis (+LVA) with codon optimization for improving expression into <i>E. coli</i>.  
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Optimized PenI repressor with LVA tag from ''Bacillus licheniformis'', with preferential codon usage for improved expression in <i>E. coli</i>. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis.
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This protein represents a negative repressor for pPenI promoter (BBa_R0074).
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Contains LVA tag.
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K566007 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K566010 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K566007 parameters</partinfo>
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<partinfo>BBa_K566010 parameters</partinfo>
 
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Revision as of 05:58, 28 September 2011

PenI repressor optimized for E. coli (inverted sequence)

Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter (BBa_R0074). The nucleotide sequence was inverted to ease DNA synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]