Difference between revisions of "Part:BBa K590061"
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<partinfo>BBa_K590061 short</partinfo> | <partinfo>BBa_K590061 short</partinfo> | ||
− | This part consists of a synthesized version of the | + | This part consists of a synthesized version of the ''luxE'' gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and is also codon-optimized for expression in ''Escherichia coli''. |
===Usage and Biology=== | ===Usage and Biology=== | ||
− | [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with [https://parts.igem.org/Part:BBa_K590059 Lux C] and [https://parts.igem.org/Part:BBa_K590060 Lux D] to engineer alternative routes for fatty aldehyde production.[[Image:Washington_luxcde_diagram.png|400px|center]] | + | The [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with [https://parts.igem.org/Part:BBa_K590059 Lux C] and [https://parts.igem.org/Part:BBa_K590060 Lux D] to engineer alternative routes for fatty aldehyde production.[[Image:Washington_luxcde_diagram.png|400px|center]] |
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Latest revision as of 05:03, 28 September 2011
LuxE; standalone, E. Coli codon optimized
This part consists of a synthesized version of the luxE gene from the [http://2010.igem.org/Team:Cambridge/Bioluminescence/Bacterial_Luciferases Cambridge 2010 LuxBrick]. It is optimized to have cloning-friendly GC content and is also codon-optimized for expression in Escherichia coli.
Usage and Biology
The [http://2011.igem.org/Team:Washington University of Washington 2011 iGEM Team] has tried to use this part along with Lux C and Lux D to engineer alternative routes for fatty aldehyde production.Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 115