Difference between revisions of "Part:BBa K566006"

Revision as of 04:10, 28 September 2011

penI-pCpcG2-pPenI-mnt

Repression-based potential AND gate. Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light (1)

Usage and Biology

This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter (BBa_R0073); so that only when green light is ON and any other Mnt source is OFF, expression from pMnt is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 992
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 912
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K566006 short</partinfo>
-'''Repression-based potential AND gate.''' Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light ([https://parts.igem.org/Part:BBa_K566005:Design 1])
+'''Repression-based potential AND gate.''' Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter. pCpcG2 promoter is positively regulated by the two component system CcaS/R, which exhibits a maximum response in 535 nm and is inactivated in 650 nm light ([https://parts.igem.org/Part:BBa_K566006:Design 1])
 ===Usage and Biology===