Difference between revisions of "Part:BBa K566000"

Latest revision as of 01:35, 28 September 2011

OL region from Lambda

OL region from Lambda phage. Allows biphasic switch (K566002) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter (K566001).

Usage and Biology

OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda (1). Its binding sites maybe used to design new regulatory networks or to mimic Lambda's natural behavior. The biphasic switch (BBa_K566002) makes a good example of that.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 2
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K566000 short</partinfo>
-OL region from Lambda phage. When placed around 2.4 kb apart from OR, allows cI octamerization and DNA looping that stabilize cI binding to OR. It makes part of the biphasic switch (K566001).
+OL region from Lambda phage. Allows biphasic switch ([https://parts.igem.org/Part:BBa_K566002 K566002]) effective repression: When placed around 2.4 kb apart from OR (included in pRM) and in high [cI] conditions, cI octamerizes and loops the DNA stabilizing cI binding to OR3; such structure represses pRM promoter ([https://parts.igem.org/Part:BBa_K566001 K566001]).
-<!-- Add more about the biology of this part here
 ===Usage and Biology===
+OL is a set of three binding sites: OL1, OL2 and OL3. It makes part of a well studied regulatory region in Lambda ([https://parts.igem.org/Part:BBa_K566000 1]). Its binding sites maybe used to design new regulatory networks or to mimic Lambda's natural behavior. The biphasic switch ([https://parts.igem.org/Part:BBa_K566002 BBa_K566002]) makes a good example of that.
 <!-- -->