Difference between revisions of "Part:BBa K566001:Design"

 
 
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<partinfo>BBa_K566001 short</partinfo>
 
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===Design Notes===
 
===Design Notes===
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Analysis of illegal restriction sites was performed, but it does not have them originally.  
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Original sequence does not have any illegal restriction sites.
  
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===Source===
  
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Obtained by PCR directly from Lambda genomic DNA (GenBank accession number [http://www.ncbi.nlm.nih.gov/nuccore/NC_001416 NC_001416]).
  
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===Source===
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===References===
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<p>1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. ''Gene Dev'' '''15''':3013–3022.</p>
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2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. ''Curr Biol'' '''9''':151–154.
  
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It comes directly from genomic DNA of Lambda phage. It was extracted by PCR as recommended by the Registry.
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<p><br></p>
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<p>'''Further Reading''':</p>
  
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===References===
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Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks.'' J Bacteriol'' '''189''':298–304.

Latest revision as of 01:22, 28 September 2011

pRM promoter from Lambda


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence does not have any illegal restriction sites.

Source

Obtained by PCR directly from Lambda genomic DNA (GenBank accession number [http://www.ncbi.nlm.nih.gov/nuccore/NC_001416 NC_001416]).

References

1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Gene Dev 15:3013–3022.

2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.


Further Reading:

Court DL, Oppenheim AB, Adhya LS (2007) A New Look at Bacteriophage λ Genetic Networks. J Bacteriol 189:298–304.