Difference between revisions of "Part:BBa K566010"
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<partinfo>BBa_K566010 short</partinfo>
 
<partinfo>BBa_K566010 short</partinfo>
  
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PenI repressor from Bacillus licheniformis (+LVA) with codon optimization for improving expression into E. coli.
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Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter ([https://parts.igem.org/Part:BBa_R0074 BBa_R0074]). The nucleotide sequence was inverted to ease DNA synthesis.
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This protein represents a negative repressor for pPenI promoter (BBa_R0074).  
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Includes LVA tag.
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Sequence was inverted for simplifying DNA synthesis.
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Revision as of 23:49, 27 September 2011

PenI repressor optimized for E. coli (inverted sequence)

Optimized PenI repressor with LVA tag from Bacillus licheniformis, with preferential codon usage for improved expression in E. coli. Negatively regulates transcription from pPenI promoter (BBa_R0074). The nucleotide sequence was inverted to ease DNA synthesis.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Unknown
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]