Difference between revisions of "Part:BBa K566001:Design"
(→Source) |
|||
Line 12: | Line 12: | ||
===Source=== | ===Source=== | ||
− | Obtained by PCR directly from Lambda genomic DNA. | + | Obtained by PCR directly from Lambda genomic DNA (GenBank accession number NC_001416). |
===References=== | ===References=== | ||
− | <p>1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny Gene Dev 15:3013–3022.</p> | + | <p>1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. ''Gene Dev'' '''15''':3013–3022.</p> |
− | 2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154. | + | 2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. ''Curr Biol'' '''9''':151–154. |
Revision as of 19:56, 27 September 2011
pRM promoter from Lambda
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Analysis of illegal restriction sites was performed, but it does not have them originally.
Source
Obtained by PCR directly from Lambda genomic DNA (GenBank accession number NC_001416).
References
1. Dodd BI, Perkins AJ, Tsemitsidis D, Egan BJ (2001) Octamerization of CI repressor is needed for effective repression of PRM and efficient switching from lysogeny. Gene Dev 15:3013–3022.
2. Révet B, von Wilcken-Bergmann B, Bessert H, Barker A, Müller-Hill B (1999) Four dimers of repressor bound to two suitably spaced pairs of operators form octamers and DNA loops over large distances. Curr Biol 9:151–154.