Difference between revisions of "Part:BBa K566006"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter [https://parts.igem.org/wiki/index.php?title=Part:BBa_R0073 | + | This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter ([https://parts.igem.org/wiki/index.php?title=Part:BBa_R0073 BBa_R0073]); so that only when green light is ON and any other Mnt source is OFF, expression from pMnt is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input. |
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Revision as of 19:53, 27 September 2011
penI-pCpcG2-pPenI-mnt
Repression-based potential AND gate. Mnt Repressor is under the control of PenI repressible promoter. PenI repressor is placed under the control of green light inducible pCpcG2 promoter.
Usage and Biology
This part is intended to be used to build a logical AND gate (or multiple input gate) to control gene expression from Mnt repressible promoter (BBa_R0073); so that only when green light is ON and any other Mnt source is OFF, expression from pMnt is allowed. The other Mnt source must then be placed under the control of a repressible promoter (other than pPenI) whose repressor expression is controlled by the second input.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 992
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 912
- 1000COMPATIBLE WITH RFC[1000]